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Human Endometrial Cancer Molecular Classification Gene Mutation Detection Kit

For Research Use only

Endometrialcancer is the most common female genital cancer in the developed world.In 2013, The Cancer Genome Atlas(TCGA) used genomics, transcriptomics and proteomics methods to analyzemutation lineage, microsatellite instability (MSI), somatic copy-number alterations, (SCNAs), TCGA proposes to divide endometrial cancer into belowcategories.

 (1) POLE ultramutated

 (2) microsatellite instability hypermutated (MSI-H

 (3) copy number low (CN-L)

 (4) copy number high (CN-H)

There are some problems for TCGA classificationsuch as multiple omics applications, difficult clinical applications, and highcosts etc. Therefore, alternative methods based on immunohistochemistry andgene sequencing have been developed.

                                                                            Nature497,67-73(2013).http://dio.org/10.1038/nature12113

Detection Item

This kit is basedon next generation sequencing method to cover the full CDS regions of PTEN,TP53, MLH1, MSH2, MSH6, PMS2 and EPCAM genes related to molecular classificationof endometrial cancer, as well as POLE exon9-14, CTNNB1 exon3 and 10 MSI loci.For the polymorphism of MSI, a double sample of both tissue and blood will be takenfor comparison. Simultaneously, KRAS and PIK3CA gene hotspot mutations will be detectedto assist classification.

Performance Parameter

Product NameCore TechnologySpecificationMatched InstrumentsSample Type

Endometrial Cancer

Molecular Classification Gene Mutation

Detection Kit

RingCap®

16 tests/kit

32 tests/kit
Ion Torrent, IlluminaFFPE ,flash tissue+ blood











Detection Significance

1 Provideprognostic information by molecular classification testing.

2 Detect MMR andMSI status to evaluate immunotherapy.

3 Screening for Lynchsyndrome.

4 The detectionof KRAS, PIK3CA, CTNNB1 genes status can be used to assist classification.


Applicable People

The detection ofmolecular classification of endometrial cancer can be used to guide theselection of treatment options.

 

Advantage

Easy Operation: With patentedtechnology RingCap®, library construction need only two steps.

Rapid Detection: Libraryconstruction need only 3.5 hours. It takes only 2 working day from sampling toreporting.
Accurate and Reliable: The sequencing depth is up to 5000X,the sensitivity can reach as low as 1%.
Comprehensive Detection:Detection of 11 genes and 10 MSIloci related to the molecular classification of endometrial cancer at one time.

 

Detection Process