Next Generation Sequencing Platform

Next-generation sequencing (NGS), also referred to as high-throughput sequencing, is capable of sequencing tens of thousands of DNA molecules simultaneously. In comparison with the first-generation Sanger sequencing, its remarkable advantages of high accuracy, high throughput, high sensitivity, and low cost have rendered it the primary detection method in precision medicine.

Principles of NGS sequencing technology

Advantages and Characteristics of SpaceGen Biological NGS Sequencing Technology

1. Simple Operation

The independent patented RingCap^® technology enables library preparation to be completed in only two steps.

2. Fast Detection

Library preparation requires only 3.5 hours.

3. Accuracy and Reliability

The sensitivity reaches as high as 0.1%.

4. Comprehensive Detection

Multiple genes can be detected simultaneously.

RingCap® technology

RingCap® (Ring-Link Capture Library Technology) is a patented technology independently developed by SpaceGen. It pertains to a targeted sequencing library preparation technology based on multiplex PCR, which addresses many limitations in the preparation of targeted libraries using traditional PCR technology, such as complex experimental procedures, long manual operation time, and high upfront equipment investment.

Core Technology—RingCap® Technical Principle

The First Stage:

1. Modified specific primers and highly specific Spacegen - Taq enzymes were employed to amplify and capture the target sequence, resulting in the formation of a sticky end in the product.

2. DNA ligase was utilized to link the asymmetrically ligated probe to the sticky end, thereby forming a circular product to prevent binding between the products.

The Second Stage:

Specific primers were used to specifically amplify the circular product, enabling the acquisition of the target sequence and, simultaneously, the binding to the required specific marker sequence.

Advantages of building based on RingCap® technical library