Scientific Research

Next-generation sequencing (NGS), also referred to as high-throughput sequencing, is capable of sequencing tens of thousands of DNA molecules simultaneously. In comparison with the first-generation Sanger sequencing, its remarkable advantages of high accuracy, high throughput, high sensitivity, and low cost have rendered it the primary detection method in precision medicine.

Quantitative Real-time PCR is a method of measuring the total amount of products after each polymerase chain reaction (PCR) cycle with fluorescent chemicals in a DNA amplification reaction. It is a method for quantitative analysis of specific DNA sequences in a test sample by internal or external parameter methods.

Digital Polymerase Chain Reaction (dPCR) is an absolute quantification technique for nucleic acid molecules. In comparison to quantitative Polymerase Chain Reaction (qPCR), digital PCR enables the direct enumeration of DNA molecules, thereby achieving absolute quantification of the initial sample.

Mass spectrometry: The components within the sample undergo ionization in the ion source, giving rise to charged ions with varying charge - to - mass ratios. An ion beam is generated via the accelerated electric field, which successively bombards the detector. Subsequently, the corresponding spectrum is collected and computed by the computer.