Detection of Septin9 methylation loci in patients with colorectal cancer.

Septin 9 gene methylation detection is a non-invasive CRC early screening, auxiliary diagnosis and efficacy monitoring technology. The detection of Septin 9 gene methylation status in blood ctDNA can assist in the diagnosis and detection of ultra-early colorectal cancer lesions, which can theoretically warn the risk of CRC 3 to 5 years in advance.

1.Healthy people over the age of 40, but have never had a colonoscopy ; 

2.High-risk groups who are not willing to undergo colonoscopy, including : 

      1) People with a family history of colorectal cancer ; 

      2) population in high-risk areas of colorectal cancer ; 

      3) People with positive fecal occult blood ; 

      4) people with changes in bowel habits ; 

      5) patients with chronic intestinal diseases ( chronic diarrhea, chronic constipation, mucous bloody stool, chronic appendicitis or appendectomy history, chronic cholecystitis or cholecystectomy history ) ;

3.Chronic diseases such as diabetes, cirrhosis, gastric ulcer, enteritis, intestinal polyps, obesity and so on ; 

4.Weight loss in the short term ; people with fever of unknown origin, pain, and mass on the body surface ;

5.Eating imbalances, people with high work pressure ; 

6.People with bad living habits such as smoking, staying up late and so on ; 

7.People after surgical treatment of colorectal cancer ; 

8.Colonoscopy taboo crowd, including but not limited to : have serious heart, liver, lung, kidney, blood, endocrine system diseases, etc.

1、Nucleic Acid Extraction

2、Modification

3、Set up qPCR

4、Amplification

5、Data Analysis